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NicholsESteinmetzJDVollsetSEFukutakiKChalekJAbd-AllahFet al
In brief, RNA was extracted from cells of the indicated genotype using the Qiagen RNAeasy RNA extraction kit (Qiagen, Manchester, UK) following the manufacturers instructions.mRNA (1 g) was retrotranscribed into cDNA using High Capacity RNA-to-cDNA kit (Life Technologies, Paisley, UK)
In addition, ESC transplantation also involves certain ethical issues
Mcl-1 regulates reactive oxygen species via NOX4 during chemotherapy-induced senescence
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