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Avoid areas with scars, bruises, stretch marks, or visible veins
The obtained PCR product included U2 promoter, a fragment encoding snRNA U12, and U2 3box and was cloned into NheI/BamHI sites of lentiviral plasmid lenti sgRNA(MS2)_puro backbone (Addgene, #73795) to generate lenti-U12 plasmid
For complete dosing math and calculations, read how to calculate peptide dosages
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Second edn, 1272 (John Wiley & Sons, 1995)
Followings are some suggested future directions to drive this field forward
