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doi: 10.1001/jamacardio.2016.0433 28 KwongJCSchwartzKLCampitelliMAChungHCrowcroftNSKarnauchowTet al

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The formazan product was solubilized with sodium dodecylsulfate (SDS, 10%, m v 1 in 20 mM HCl), and the absorbance was measured at 595 nm relative to the absorbance of vehicle (DMSO)-treated control cells using a multiwell scanning spectrophotometer (Victor 3 plate reader, PerkinElmer, Rodgau-Juegesheim, Germany)

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One of the derivates of Daidzein the 8-Hydroxydaidzein can also play a role as an anti-inflammatory compound in activated macrophages such as RAW 264.7 cells by controlling the proinflammatory cytokines and NF-B pathway, suggesting that Daidzein could block DSS-induced UC and reducing inflammatory factor expression [186]