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In a case study on the effects of changing the pH of numerous drugs of abuse, it was discovered that even a single pH unit change could result in significant locomotor alterations in zebrafish at a constant drug concentration (Cleal et al., 2020)

doi: 10.1038/s41388-019-0873-8

doi: 10.1016/0196-9781(92)90078-H

Liao C, Nicklaus MC (2009) Comparison of nine programs predicting p \( {\mathit{K _ \rm a \) values of pharmaceutical substances

Overall, PIs are expected to include the true intervention effect expected in future studies, and they incorporate an extra component of variance, specifically between-study heterogeneity