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Description
(2)Fmoc-Leu-Val- (2) Preparation of Fmoc-Leu-Val-resin: (1)Fmoc-Val-1050530105010603DMF3DMF2DMFFmoc-Leu-OHDIEATBTU/HOBtHBTU/HOBtBOP/HOBt105010603DMF3DMF2Fmoc-Leu-Val- In the Fmoc-Val-resin of step (1), add decapping reagent 1050 and react for 530 minutes, drain with vacuum pump, add decapping reagent again 1050 and react for 1060 minutes, decapture, use iso Wash 3 times with propanol, 3 times with DMF, 2 times with distilled DMF, add the mixture of Fmoc-Leu-OH, DIEA, TBTU/HOBt or HBTU/HOBt or BOP/HOBt dissolved in DMF, 10-50 React at for 10-60 minutes, dry with a vacuum pump, wash with isopropanol for 3 times, DMF for 3 times, DMF that has been re-distilled for 2 times, and dry to obtain Fmoc-Leu-Val-resin
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Therefore, since then, this AIFM2 oxidoreductase has been re-named to Ferroptosis Suppressor Protein-1 (FSP1) (Figure 2)
