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Despite these advantages, many current HILIC methods still rely on chemical derivatization to achieve adequate sensitivity [48, 59,60,61] or depend entirely on MS/MS specificity while neglecting optimal chromatographic separation, which can lead to severe matrix interferences and ion suppression [41]

When liver cells are stressed, they leak ALT into the bloodstream

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Characteristics of included studies Among the included trials, 119 (45.4%) were randomized controlled trials (RCTs) and the majority (80.5%) focused on adult populations