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Histone demethylating agents as potential S-adenosyl-l-methionine-competitors

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While the physiological pathways MOTS-c influences, such as the AMPK pathway, are highly conserved across species, the pharmacokinetics and ultimate clinical efficacy can differ significantly between mice and humans

After transfer of proteins onto nitrocellulose, membranes were Western blotted with the primary antibodies specified below

The wild-type exposed to alkaline phosphatase and the mutant enzyme had higher activity compared to the wild-type enzyme not treated with phosphatase, and they were also less sensitive to inhibition by SAM [22]
