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Description
Delpire, E
Swenson ER
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The pelleted cells were washed twice with 0.85% NaCl, resuspended in 1 mL 30% methanol/water, and lysed on ice with ultrasonication
Check for any particulate matter, cloudiness, crystallization, or color changes from previous doses
The fear of looking frozen or overdone is real, and valid but its almost always a consequence of poor technique rather than the treatment itself
