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Cells were extensively washed with PBS, fixed in 4% PFA for 15 min, permeabilized and blocked with 0.01% saponin/10% normal goat/PBS serum and stained with the primary antibodies in the same blocking solution in a humid chamber

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Once reconstituted, the clock starts ticking

9.Anemia associated with bone marrow disease Diseases such as leukemia and bone marrow cancer (Myeloma) can affect how the bone marrow produces blood, and treatment may include some medications, chemotherapy, or a bone marrow transplant
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