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Stoichiometry of the TCA Cycle The overall stoichiometry of the TCA cycle is: acetyl-CoA + 3NAD + + FAD + GDP + P i + 2H 2 O 2CO 2 + 3NADH + FADH 2 + GTP + 2H + + CoASH Role of Lactate as Driver for TCA Cycle Activity The lactate produced by anaerobic glycolysis does not simple serve as an intracellular carbon source for hepatic glucose production via gluconeogenesis

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