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They identified that a 40% coverage of polyclonal antibodies linked to a SAM with 10% carboxyl groups provided optimal sensitivity, achieving a detection limit of 5.9 1,065.9 \times 10^65.9 106 EVs/mL across a linear range of 1.9 1,081.9 \times 10^81.9 1081.9 1,091.9 \times 10^91.9 109 EVs/mL

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Calculations were performed with the Seahorse Mito Fuel Flex Test Report Generator plug in for excel and inhibitor injections essentially followed manufacturer protocols using sequential or combined injections of etomoxir, BPTES, and UK5099

erinaceus were in the range of 5.09.0, with pH 6.0 as optimal

At least seven additional vendors closed throughout 2025