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The cells were lysed in lysis buffer [10 mM Tris (pH 7.4), 10 mM KCl, 2 mM MgCl, 1 mM EGTA, 1 mM DTT, 0.05% (v/v) Triton X-100 and 1 protease inhibitor (Pierce)] for 30 min on ice

Proietti S, Cucina A, DAnselmi F et al (2011) Melatonin and vitamin D 3 synergistically down-regulate Akt and MDM2 leading to TGF-1-dependent growth inhibition of breast cancer cells

Wang R, Liu ZH, Bi N, Gu X, Huang C, Zhou R, et al

doi: 10.1212/WNL.42.3.631 5 Arruebarrena Di PalmaA.PerkE

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Therefore, we reported evidence about blood vessel recruitment and activation (running) toward the site of injury, also described as bypassing occlusion via alternative pathways (Vukojevic et al., 2018