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Payen D, Lukaszewicz A-C, Belikova I, Faivre V, Gelin C, Russwurm S, et al

13 , e034731 (2024)

In addition, we showed that GSH depletion induces autophagy and SIPS, with autophagy being a negative regulator of SIPS

Protein preparation and Western blot analysis Cultured cells or flashfrozen tissues were homogenized in icecold radioimmunoprecipitation assay (RIPA) buffer (50 mM TrisHCl pH 7.6, 150 mM NaCl, 0.5% sodium deoxycholate, 1% NP40, 0.1% SDS, 2 mM EDTA) containing COMPLETE protease inhibitor cocktail (Roche) and phosphatase inhibitors (Santa Cruz Biotechnology)

Rabin et al., 1993