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Furthermore, amplicons were purified prior to sequencing by the NucleoSpin Gel and PCR Clean-Up kit (MachereyNagel, Dren, Germany)

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Many of the studies that implicate axonal involvement in glaucoma have been conducted in optic nerve crush models that are not directly relevant to pressure-induced axonal injury

doi: 10.1016/j.plefa.2007.08.007 42 ChenCJHuangHSLinSBChangWC
