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There are two 2HG enantiomers: D-2-hydroxyglutarate (D2HG) and L-2-hydroxyglutarate (L2HG), with the former predominantly generated from IDH1 mutants and PHGDH while L2HG is primarily generated from the promiscuous activity of LDHA and MDH1/2 under hypoxic or acidic conditions and converted back to 2OG by L2HGDH

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The decrease in GSH disrupted the REDOX balance, preventing the consumption of intracellular ROS and lipid peroxides, and contributing to oxidative stress in cells, manifesting as a decrease in antioxidant proteins and the appearance of DNA damage

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EtOH/POA also promotes calcium overload and nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase activation

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