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Research data indicate that patients with CP/CPPS often exhibit a significantly reduced proportion of morphologically normal sperm and an abnormally elevated sperm DNA fragmentation index (DFI) (with some studies reporting a median of 16.0%, nearly double that of the control group at 8.4%) ( Figure 2 )

supernatant was used to produce an enzyme and assess the activity of specific enzymes after using the same buffer to dilute it to a certain amount

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