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Altering kinetic mechanism and enzyme stability by mutagenesis of the dimer interface of glutathione reductase

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Then, 500 L of 20 mg/ml BSA (bovine serum albumin) was added to the lysate before a second filtration step with a new filter with a 40 m mesh size, followed by a third filtration with a mesh size of 10 m

For aging, animals were aged at 25C and treated with Lip-1 or SIH as previously described