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These states include the rotation of the 30S (SSU) with respect to the 50S (LSU) (127, 135138), translocation of the transfer RNAs and mRNA during the reverse rotation of the 30S subunit by the elongation factor G (EF-G) (139146)

For lyophilized powder, assume 48 hours at room temperature (acceptable risk)

Indian Journal of Clinical Biochemistry , 32 , 134-141

The second and third hotspot are known as the HGF chain, localized on blade five of the SEMA domain and immunoglobulin-plexin-transcription factor (IPT) homology domains 23, respectively

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