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doi: 10.1210/clinem/dgz289 [DOI] [PubMed] [Google Scholar] 50

The National Center for Biotechnology Information reviews how NAD+ functions as a coenzyme in metabolism and cellular signaling, which is the biology these protocols aim to support

Each RT-PCR reaction consisted of 10 L of SYBR Green Supermix (Bio-Rad, iTaq Universal SYBR Green Supermix, 1725124), 7.6 L of distilled water, 0.4 L of primer mix, and 2 L of cDNA
