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Pulldown assays with recombinant eIF2B dimers ( 2 ), eIF2B tetramers () or eIF2B decamers (() 2 ) in vitro indicated that MBPAcP10 bound directly only to eIF2B decamers (Fig

Antibodies, inhibitors and cytokines Antibodies used in flow cytometry were purchased from BioLegend (Koblenz, Germany), including APC anti-MICA/B (6D4), PE anti-PD-L1 (MIH2), APC anti-CD86 (BU63), PE anti-CD206 (15 2), anti-CD16/32 (93), APC anti-PD-1 (EH12.2H7), FITC anti-NKG2D (1D11), PE anti-p38 MAPK Phospho (A16016A), PE anti-STAT3 Phospho (13A31), APC anti-NF-B Phospho (14G10A21), PE anti-ERK1/2 Phospho (6B8B69), APC Mouse IgG2a, (MOPC-173), PE Mouse IgG1, (MOPC-21), APC Mouse IgG1, (MOPC-21), and PE Rat IgG2a, (RTK2758)

[DOI] [PubMed] [Google Scholar] 109.Kim M.G., Pak J.H., Choi W.H., Park J.Y., Nam J.H., Kim J.H

Sigma-Aldrich) coated plates

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