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The resolving electron donor thiol can be glutathione, allowing the formation of a mixed disulfide, while the second donor thiol enables the reduction of the formed disulfide bonding

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Prepare small aliquots to avoid repeated freeze-thaw cycles

Similarly, Subramani and Ganapathyswamy highlight that lipid-based carriers like liposomes are a superior choice for encapsulation of sensitive ingredients of varied polarity- covering hydrophobic and hydrophilic bioactive molecules alike (29)