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This latter observation is strong evidence that adipocytes play a major regulatory role in the HBP-mediated whole-body insulin resistance

Cultivation was continued for 48 h, and then a 2 mL sample was drawn from which the cell pellet and supernatant were obtained by centrifugation (10 min, 12,000 g , 4C)

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Under conditions of ceramidase inhibition there is an exaggerated effect of palmitic acid addition on insulin resistance