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Millipore), and images were acquired using the ChemiDoc Touch imaging system (17001401JA
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this compound is autocyclized to form 5-OP when there is an insufficient amount of Cys as a substrate, as discussed above in the development of 5-oxoprolinuria
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The oxidized form of glutathione (GSSG) is reduced to GSH using DTT reagent, allowing for determining total glutathione content, including both oxidized and reduced forms
