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The samples were subjected to heat treatment at temperatures ranging from 40 C, 50 C, 60 C, 70 C, 80 C and 90 C for 3 min, followed by a 3-minute cooling period at 4 C in a thermocycler

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The sample to be tested is labeled and hybridized with the probes, determining the presence and expression levels of DNA/RNA in the sample through signal detection (Zhu et al., 2006)

A newer solution including AAs, niacinamide, coenzymes, glutathione, and HA was effective in repairing the epidermal basement membrane, reducing oxidative stress, and managing aging-related factors, thereby enhancing skin elasticity and collagen accumulation for rejuvenation