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To understand the function of those genes, in addition to using the existing genome annotation, we further annotated the genes by blasting their protein sequences against Uniprot database, using PANNZER2 (Trnen et al., 2018) and local BLAST (Altschul et al., 1997

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doi: 10.3389/fphar.2023.1094435 109

In patients with longer disease duration, the abundance of Fournierella and DTU089 (Ruminococcaceae) increased, while that of Roseburia (Lachnospiraceae) decreased

Worked example: 10 mg vial with 2 mL water Concentration: 10 mg divided by 2 mL = 5 mg/mL For a 2.5 mg dose: 2.5 divided by 5 = 0.50 mL = 50 units For a 5 mg dose: 5 divided by 5 = 1.0 mL = 100 units For a 7.5 mg dose: 7.5 divided by 5 = 1.5 mL = 150 units (requires a larger syringe or two draws) Worked example: 30 mg vial with 3 mL water Concentration: 30 mg divided by 3 mL = 10 mg/mL For a 2.5 mg dose: 2.5 divided by 10 = 0.25 mL = 25 units For a 5 mg dose: 5 divided by 10 = 0.50 mL = 50 units For a 10 mg dose: 10 divided by 10 = 1.0 mL = 100 units For a 15 mg dose: 15 divided by 10 = 1.5 mL = 150 units Notice how the 10 mg/mL concentration makes the math cleanest

Moreover, the cell proliferation ability of HCC cell lines (MHCC97H and HepG2) and PCa cell lines (LNCaP and C4-2) increased with an increase in Gln concentration, as determined by cell CCK- 8 and colony formation assays (Fig