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Subsequently, slides were washed 3X with PBS (10 min, RT) and incubated with the secondary horseradish peroxidase conjugated antibody (mouse anti-rabbit IgG, 0.4 mg/mL diluted 1:500, sc-2357 or goat anti-mouse IgG, 0.4 mg/mL diluted 1:500, sc-2031) for 2 h at RT, followed by three PBS washes and treated with the 3,3-diaminobenzidine substrate (ab64238, Abcam) for 10 min

Some patients benefit from switching to a low-dose maintenance plan rather than stopping abruptly

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Clinical conditions associated with glutathione depletion include increased oxidative stress and dysfunction of enzymes within the -glutamyl cycle

SL route could be used in patients with pernicious anemia or malabsorption causes

Even though most of these techniques are in experimental stages only and need to go under clinical trials and evaluation, among all these emerging technologies, nanotechnology-based nanomedicines are gaining popularity among researchers for the theragnostic purposes of PD (16)