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The microinjected cells and EV-treated cells were fixed by 4% PFA for 5 min at room temperature and stained by rabbit anti-Halo-tag antibody and Alexa-647-labeled phalloidin

Clinical response correlates strongly with early intervention

This method injects the peptide directly into the abdominal cavity

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Imaging studies demonstrate that while the number of presynaptic contact sites may remain unaffected or even increase following Pb exposure (e.g., increased bassoon immunoreactivity), the quality and functionality of these sites are diminished, as evidenced by decreased vesicular protein co-localization and a decline in fast-releasing vesicle populations [102]

Some patients may experience minor redness or tenderness at the injection site