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On the day of the assay, plates were removed from the incubator and each well was washed twice with 100 l HBSS (Gibco, Life Technologies) and pre-incubated for 30 min at 37 C with 85 l HBSS per well

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192 Moreover, lowering Cu levels using TTM affects the activity of MEK1/2 kinase and BRAF-driven tumorigenesis, thus decreasing the growth of xenografted BRAF V600E tumors

700 Activation of PPAR was also reported for 9-oxo-OTrE, resulting in enhanced fatty acid uptake in murine hepatocytes

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