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Insulin receptors are abundant in the brain, however, concentrated in some areas such as the substantia nigra and basal ganglia

After centrifugation for 15 min at 12,000 g , a 120 l supernatant was mixed with 100 l each of solution 1 (containing 100 mM phenol and 50 mg l 1 sodium nitroprusside) and solution 2 (comprising 0.38 M dibasic sodium phosphate, 125 mM NaOH, and 1% sodium hypochlorite with available chlorine ranging from 10 to 15%)

109,110 Additionally, antioxidants including vitamins A and C, CoQ10, and -lipoic acid have been suggested to improve behavioral parameters and reduce oxidative stress, mitochondrial dysfunction, and glutamate-induced toxicity in PD

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Therefore, overcoming the obstacles in biofilm therapy is one of the main challenges in the treatment of biofilm-related diseases