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Excessive fat intake has previously been demonstrated in mice to increase intestinal permeability, confirmed by high levels of circulating gut-derived LPS and reduced TJ protein expression (Cani et al

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81 Additionally, CB2R knockout mice exhibit gut flora dysbiosis similar to that of NAFLD patients and model mice

Supernatant was recovered following centrifugation at 16,000 g for 10 min at 4 C, and protein was quantified using the Pierce BCA protein assay kit (Thermo, Australia), according to manufacturers instructions

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