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BT16 cells were treated with the glutathione inhibitor buthionine sulfoximine (BSO) which inhibits gamma-glutamylcysteine synthetase, the enzyme required in the first step of glutathione synthesis (Tagde et al

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Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for GSH-PX is added to each Microelisa stripplate well and incubated

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The outcomes of these studies were that curcumin could affect performance and the inflammatory response throughout short-term interventions, but more well-controlled investigations are still needed