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Metabolic flux by U- 13 C-glutamine tracing measurement Cells in 10-cm dishes were washed twice for metabolite tracing by glutamine-free RPMI-1640 medium

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AMPK regulates aging through integrating multiple key cell signals, including AMPK-mTOR, AMPK-Nrf2, AMPK-SIRT1 and other regulatory networks (Fig

Quantification of a Panel of Inflammatory Cytokines The levels of the anti-inflammatory cytokine IL-10 and the pro-inflammatory cytokines TNF-, IL-6, and IL-1 in brain cell homogenates were quantified using specific ELISA kits (Ray Biotech, Peachtree Corners, GA, USA) according to the manufacturers instructions

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Because they can interact with specific receptors, they can have strong biological effects even at low doses