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As A2ARi induced better effector function of CD8 + T cells along with more ferroptosis, we then combined A2ARi and Lip-1 to test whether combination therapy would induce superior responses in CD8 + T cells

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Further analysis indicated that the wounds exposed to GHK might have shown elevated levels of glutathione and ascorbic acid

The cells were incubated with primary anti-PD-L1 (1:200, A1645, ABclonal, RRID: AB_2763702) overnight at 4 C, followed by incubation with Cy3-labeled goat anti-rabbit IgG (1:500, A0516, Beyotime) for 60 min at RT in the dark