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Oct 2012;11(5):876-884

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indeed, the nature of the initial experiments, utilizing either microsomal fractions or cells whose plasma membranes had been permeabilized to allow access of the inositol phosphate, precluded investigation of mechanisms of Ca 2+ influx
( A ) Total fat stores in mutant worms relative to wild type worms assayed by feeding worms with vital dyes Nile Red and BODIPY-labeled fatty acids, with CARS imaging, and with fixative staining with Nile Red