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These metabolites are generated by bacteria such as Clostridium and Desulfovibrio , which are frequently enriched in PD [25]

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After acidification with trifluoroacetic acid 1% (TFA) and centrifugation at 16,000 g for 5 min, resulting peptides were purified on StageTips according to Rappsilber et al

Tumor necrosis factor-alpha (TNF) production is increased in adipocytes from obese individuals and may contribute to enhanced basal lipolysis in obesity (97, 104, 105)
