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doi: 10.3390/ijms19092745
Br J Haematol 161(3):357-66, 2013
The sgRNA used to generate GFP-DME also targets the 3 homology region of the donor construct, so we introduced silent mutations within the 3 donor sequence of GFP - DME to prevent sgRNA binding, DSB and mutations following precise knock-in (Supplementary Fig
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We recommend a minimum of 3 sessions in order to achieve effective results
In contrast, the levels of pro-ferroptotic PEs and ePEs with various PUFA chains were markedly reduced in MBOAT2-overexpressing cells, leading to ferroptosis resistance 97
