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Treatment of cells with supplemental iron results in an increase in the HIF hydroxylase activity, while iron chelation produces the opposite effect ( Cultured human cells lacking PCBP1 or PCBP2 exhibit nuclear accumulation of active HIF1, which increases in cells made mildly or transiently iron deficient ( The activity of FIH1, also an iron- and oxygen-dependent HIF1 regulator, is dependent on PCBP1 as well ( Other classes of iron metalloenzymes may also depend on iron delivery by PCBPs