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Similarly, total protein lysate (uptake fraction) from cells in the second plate was isolated after 4 h using the same protocol

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All GSH wash 2, elution, and strip buffers contained 1 mM dithiothreitol (DTT) (Thermo Fisher Scientific)

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JCI Insight 2:e90133

MK-677, also known as ibutamoren, is an orally active small-molecule growth hormone secretagogue (GHS)