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doi: 10.1002/(SICI)1097-0231(199704)11:6 3.0.CO;2-O

After pharmacological intervention in 96-well cell culture plates for 24, 48 or 72 h, the culture medium was removed and replaced with 0.1 mg MTT reagent (Beyotime, ST1537) per well dissociated in 100 L basal DMEM (Procell, PM150210)

Growth can range from very slow to explosively fast and is associated with significant mortality and morbidity

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