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Figure 3 3.1.3 EGCG anti-neuroinflammatory activity in AD Neuroinflammation as a pathogenesis of AD has been confirmed by numerous studies

Liver samples were then mixed with 4X Laemmli sample buffer (8% SDS, 40% glycerol, 0.4% bromophenol blue, 240 mM Tris, pH 6.8)

Dissociation of acini from pancreatic tissue Functional intact acini were dissociated from pancreatic tissue using collagenase digestion with mild shearing forces as previously demonstrated 44

The released ATP then interacts with surface receptors, leading to pore formation in the membrane and cell death through a mechanism resembling mitochondrial apoptosis [52]

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