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Quantitative analysis for copper, by atomic absorption analysis on fresh hepatic tissue, is the definitive method to document increased hepatic copper content

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When HeLa cells are treated with Ero1 inhibitor (EN460), ER oxidative protein folding is impaired, reducing H 2 O 2 signaling and inhibiting Nrf2 translocation to the nucleus, resulting in a decrease in GPX8, resulting in increased ER calcium content and signaling (Granatiero et al., 2019)

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Increased hepcidin levels in preeclampsia: a protective mechanism against iron overload mediated oxidative stress