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Methods Study design This was a 12-week, prospective, single-arm, open-label, phase IV multi-center trial

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MS-grade Trypsin (Thermo Scientific, #90057) was reconstituted in 20 mM acetic acid and added to cell samples at 1:100 (g trypsin:g total cellular protein) dilution before overnight incubation at 37 C and then acidification with 0.5% formic acid (FA)