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At 0, 3, 7, and 10 days, the tissues were fixed, and cryosections were used for in situ apoptotic staining with terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL), according to the manufacturers instructions (Servicebio, Beijing, China)
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During planning of the original analysis, a stepwise approach was judged to be superior to a joint estimation of all data (s.c., i.v
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