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Crucially, the researchers explored substituting these expensive growth factors with a small-molecule cocktail, specifically 10 g/mL Vitamin C, 0.1 M Dihexa, and 10 M Forskolin (VDF cocktail), to assess its efficacy in inducing hepatic specification

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These include reductions in brain volume (particularly within the prefrontal cortex and hippocampus), grey matter atrophy, decreased secretion of neurotransmitters such as acetylcholine and dopamine, and impaired synaptic efficiency (Yang et al., 2023)