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Fat loss occurs when: Energy expenditure exceeds energy intake Hormonal pathways support nutrient partitioning Lean mass is preserved Research consistently shows that peptide effectiveness is amplified when combined with: Structured resistance training Protein-adequate diet Sleep optimisation Sustainable caloric control They are modulators not replacements for foundational lifestyle variables

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Horseradish peroxidase-conjugated secondary antibodies and an enhanced chemiluminescence substrate kit were used for the detection of specific proteins