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Pipette 5 mL of the above solution into a 100 mL conical flask, add another 5 mL of 2% HPO solution to make a total volume of 10 mL, then add 1 mL of 5% potassium iodide and 2 drops of starch indicator

After mixing, the absorbance was recorded at 517 nm on a UV-1600 PC VWR spectrophotometer (VWR, China)

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