glutathione is nucleophilic or electrophilic catalysis and the reaction mechanisms of glutathione-dependent enzymes Phase II - Glutathione Conjugation
Description
If it is more than 0.1 or 0.2 pH units away from your desired pH, then youll want to use concentrated NaOH to adjust the pH of your elution buffer
A representative picture of DED is demonstrated in Fig

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[DOI] [PubMed] [Google Scholar] 88.Zasloff M et al

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