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Description
Lyophilization helps maintain structural stability by removing moisture from the peptide formulation
After living in Plano, Texas, and later returning south, David settled in Mobile, Alabama, which he has called home for the past 30 years
, DSIP , WADA
The digestion was stopped by addition of 100 l of 2 STOP buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA, 0.005% digitonin, 100 g ml 1 RNase A and 50 g ml 1 glycogen)
Trichloroacetic acid (TCA, 10%) was added to the homogenate, the mixture was centrifuged at 3000 rpm for 5 min, and the supernatant was collected
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