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5-FU is an anti-metabolite agent that inhibits thymidylate synthase and incorporates its metabolites into DNA and RNA, leading to DNA damage and cancer cell death (43)

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Shulan H., Peng N., Likun L., et al., Effectiveness of Yiqi Chupi Powder for Alleviating Cancerrelated Fatigue in Patients Following Colorectal Cancer Surgery: A Randomized Controlled Trial, Journal of Traditional Chinese Medicine 45, no

All real-time PCRs were performed three times and all changes in gene expression were reported as multiples of increases relative to the control while GAPDH was used as an internal control and relative gene expression between the experimental groups were determined using MxPro software

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