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To allow uncoupled respiration, 40 M DNP (2,4-dinitrophenol) was added

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The AS01 B adjuvant suspension is composed of 3- O -desacyl-4'-monophosphoryl lipid A (MPL) from Salmonella (Minnesota strain) and a saponin molecule (QS-21) purified from Quillaja saponaria (soap bark tree) extract, combined in a liposomal formulation consisting of dioleoyl phosphatidylcholine (DOPC) and cholesterol in phosphate-buffered saline solution